FTIR Spectrum and Antimutagenicity of Coffea arabica Pulp and Arachis hypogaea Testa In Relation to their In Vitro Antioxidant Properties

Coffee pulp (Coffea arabica) and peanut testa (Arachis hypogaea) are common agricultural wastes which are extensively studied as potential sources of bioactive compounds. This study attempted to determine the correlation of the antioxidant potential, lipid peroxidation inhibition and antimutagenic activities of the crude extracts of both plant samples. Quantification of phenolic compounds, flavonoids and monomeric anthocyanins and Fourier Transform Infra-red (FTIR) analysis were performed to characterize bioactive compounds. Based from the results, peanut testa extract (PTE) exhibited higher total antioxidant capacity (TAC) compared to coffee pulp extract (CPE) at < 500μg/mL. Higher lipid peroxidation inhibition (LPI) was noted in CPE at > 500 μg/mL but was lower than L- Ascorbic Acid (LAA). The antimutagenic activity of PTE at > 125 μg/mL was higher compared to both RPE and LAA. Mitotic indices of A. cepa treated with PTE+MMS were higher compared to groups treated with RCPE+MMS, LAA+MMS, deionized water and MMS only. Correlation analysis revealed the TAC of ripe coffee pulp extract had significant moderate correlation with its antimutagenic activity but high correlation with LPI. The LPI of CPE showed significant moderate correlation with its antimutagenic activity. In PTE, TAC shows high significant correlation with its antimutagenic property and LPI while LPI has significant moderate correlation with its antimutagenic property. The observed activity of the CPE and PTE may be attributed primarily to the phenolic compounds in both plant extracts.

Keywords – antimutagenicity, antioxidant capacity, Arachis hypogaea testa, Coffea arabica pulp, FTIR