Rhea Lyn S. Arangusti, Justine Ann F. Crucena,
Vianca Marie P. Dorado, Judy Ann C. Magsino,
Antonio F. Laude, Jr. , Cesar M. Mendoza, Jr.
Student researcher, School of Medical Technology, Emilio Aguinaldo
College-Manila 5Faculty 6Dean, School of Medical Technology,
Emilio Aguinaldo College-Manila
rlangusti@gmail.com, jacrucena23@gmail.com,
viancadorado@gmail.com, judyannmagsino@gmail.com,
tonix.laude@eac.edu.ph, cesar.mendoza@eac.edu.ph
Date Received: June 6, 2019; Date Revised: September 2, 2019
Asia Pacific Journal of Multidisciplinary Research
Vol. 7 No.4, 55-60
November 2019
P-ISSN 2350-7756
E-ISSN 2350-8442
www.apjmr.com
CHED Recognized Journal
ASEAN Citation Index
Carmona Retusa (Tsaang gubat) Roots and Stem Methanolic Extract: Anti-Angiogenic Effect 528KB 3 downloads
Rhea Lyn S. Arangusti, Justine Ann F. Crucena, Vianca Marie P. Dorado, Judy Ann C....
Cancer is one of the leading causes of death worldwide. Angiogenesis is a factor for cancer progression and most researches focuses on anti-angiogenesis to inhibit the metastasis of cancer. The purpose of the study was to screen the anti-angiogenic effect of Carmona retusa roots and stem crude methanolic extract using chorioallantois membrane assay on fertilized duck embryo. A total of 7 mL were obtained from the stem extract and 3 mL for the roots extract and were diluted with distilled water obtaining concentrations of 25 g/mL, 50 g/mL, 75 g/mL and 100 g/mL. Experimental design was used in the study.
Treatments were prepared in three trials. Extracts were applied in ovo without breaking the chorioallantois membrane (CAM) of duck embryo. Blood vessels were viewed under a stereomicroscope and photomicrographs were taken. Branching points were counted through NIH Image J software and results were submitted to a Two-way analysis of variance. Study showed that C. retusa roots and stem extract do not have significant difference F(1,21)=.016=p=.960 in anti-angiogenic effect. Administration of C. retusa roots and stem in different concentration showed significantly F(3,21) =6.029=p=.004 anti-angiogenic
effect on the branching points of the blood vessels. A higher concentration of the extract (roots and stem) would have a greater effect in lessening the blood vessel on the CAM of fertilized duck embryo. C. retusa roots and stem methanolic extract demonstrates to have an anti-angiogenic potential.
Keywords: Angiogenesis, Chorioallantoic membrane assay, NIH Image J, methanolic extract